金属錯体の逆相高速液体クロマトグラフィーの高性能化に関する研究

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Reversed phase-high performance liquid chromatography (RP-HPLC) of metal chelates with azo dyes was investigated for the selective separation of metal ions and was applied to the determination of trace amounts of metal ions. This method was based on the precolumn derivatization of metal ions with azo dyes, and the subsequent separation of the metal chelates using an RP-column and spectrophotometric detection of the chelates. Two novel techniques were proposed to develop the selective separation of the metal chelates. The first was the use of tetraalkylammonium salts (TAASs) in aqueous organic solvent mobile phase ; the second was to employ an aqueous micellar mobile phase containing nonionic surfactant. In the former method, TAAS was found to be very effective for decreasing the retention time of the chelates including positively charged chelates strongly retained on the RP support. In the latter method, the retention time in RP-HPLC using a micellar mobile phase (micellar RP-HPLC) was successfully controlled by varying the oxyethylene chain length of the nonionic surfactants, in addition to adjusting the concentration of the surfactant. Moreover, the two techniques were combined to develop the rapid and selective separation of a cationic Co (III) chelate with azo dye. The combined use of TAAS with micellar RP-HPLC would provide a useful means for enhanced selective separations of many compounds. These methods have been applied to the determination of trace amounts of V (V), Co (II), Ni (II) and Fe (III) in high-purity silicon and environmental samples ; the detection limits of V (V) and Co (II) were 2.2 pg and 0.72 pg in 100 mm^3-injection, respectively.
社団法人日本分析化学会の論文
1995-03-05

金属錯体の逆相高速液体クロマトグラフィーの高性能化に関する研究

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