大型回分培養槽による炭化水素からの菌体生産 : 炭化水素からの菌体生産(第2報)

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In a previous report, the basic culture conditions of Candida yeast (Y-183), having 0.25 hr^<-1> of μ_<max>, were studied. In this paper, the relation between cell growth and dissolved oxygen (DO), the oxygen demand for yeast cell production was investigated using a 500 l-Waldhof type fermenter for batch cultivation, and also, the growth pattern in the n-paraffin medium was compared with that in a glucose medium. The conditions of culture were as follows : Working volume of medium : 220 l Conc. of m-paraffin : 1-4 wt.% Temperature : 30℃ Agitation speed : 900 rpm Air flow : 1 vvm pH : 4 (adjusted with 28%-NH_4OH) Defoaming was done by means of spraying the culture broth to the surface using a circulating pump. The contents of O_2 and CO_2 in the exhaust were recorded continuously by a gas analyzer. The specific growth rate was found to be 0.25hr^<-1> in the logarithmic phase, but showed a tendency to decrease in concentration at a DO of less than 2 ppm. The yield of yeast cells for weight of n-paraffin was in the range of 95〜97%, and 1.5 g of oxygen was consumed for 1 g of yeast cell production. The respiratory quotient (CO_2/O_2) was determined to be 0.5 in the n-paraffin medium and 1 in the glucose medium.
社団法人日本生物工学会の論文
1973-07-25