石油酵母培養廃水の活性スラッジ処理およびスラッジ抽出液の利用 : 炭化水素からの菌体生産(第3報)

概要

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A continuous activated sludge process for the treatment of the waste from Candida yeast culture-broth and utilization of the extract from the resulting sludge for yeast cultivation were investigated in order to reduce the running cost of biomass production from hydrocarbon. The results obtained were as follows : 1. Activated sludge treatment was carried out in the range of BOD loading : 2.19〜6.84 g/l/day at 25℃ using an aerator with a 4 l capacity. The maximum loading of BOD was found to be 5.47 g/l/d with a 97% BOD reduction, and under these conditions, 30% of excess sludge per removed BOD in weight was formed. 2. The effect of activated sludge-extract as an organic nutrient on yeast growth was tested. The sludge extract was made from centrifuged sludge cake (water content : 94%) by means of autolysis and extraction by boiling. After extraction, 20 ml of extracted solution was obtained from one gram of dry sludge by filtration. The filtrate was added to a synthetic medium, containing 1% n-paraffin, to the extent of 0.2〜2%, and shaking culture tests were conducted. The addition of sludge extract was concluded to be effective on the cell growth as an organic nutrient and could be used for this purpose instead of expensive yeast extract or corn steep liquor.
社団法人日本生物工学会の論文
1973-07-25