炭化水素からの菌体生産 : (第1報) 酵母培養の基礎的条件

概要

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A soil-isolated yeast, Y-183,was cultivated to investigate the growth of the organism on hydrocarbon media in shaken flasks. Normal paraffin in the range of n-tetradecane to n-octadecane, containing about 75% of n-pentadecane, was mainly used in this study. The highest specific growth rate, μ=0.25hr^<-1>, was obtained at 30℃ independent of the initial concentration of n-paraffin. The cell yield was nearly 90% at 27-32℃ and 95% at 20-24℃. The temperature over 35℃ emarkably lowered the growth rate and cell yield. Extracts of activated sludge from alcohol fermentation waste were available as a kind of organic nutrient instead of expensive yeast extract. The high content of iso-tridecane in tridecane had scarcely any influence on the growth of the yeast. A few experiments were conducted with additions of some surfactants to examine their effects on the cell growth. One of them, namely polyoxyethylenepolypropylene monostearate, did not practically deteriorate the cell growth, and in this case a good contact between the cells and the oil droplets in the medium was observed microscopically.
社団法人日本生物工学会の論文
1970-05-25