微生物呼吸反応の自動測定

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The amount of oxygen consumed in the respiration of microorganisms was measured by an automatic apparatus.The apparatus consisted of a reaction chamber, a master controller, a measuring unit, and a recorder. The reaction chamber included a culture flask with a magnetic stirrer and a small vessel for the carbon dioxide absorbent, a manometer for sending a signal of pressure change to a relay circuit and an electrolytic oxygen generator with a copper sulfate electrolyte. The oxygen consummed in the respiration of microorganisms is fed from the oxygen generator. During the eletrolysis a synchronous motor is rotated at a constant rate. The potentiometer is directly connected to the synchronous motor and the curve of oxygen consumed is recorded on the chart as an mV-time curve. (Respirometers of this type are now being placed on the market in Japan.)In this paper, some experiments on the respiration of activated sludge and Candida yeast were conducted using this respirometer. In the experiments on the activated sludge, influence of inhibitors, the biochemical oxidation of some organic compounds, and the ratio of O_2 uptake to weight of increased or decreased sludge were investigated. On the Candida yeast, the ratio of O_2 uptake to cell weight in glucose and n-paraffin medium, measurement of the specific growth rate, and the effect of yeast extract on growth were investigated through the oxygen consumption curve.
社団法人日本生物工学会の論文
1972-05-25

微生物呼吸反応の自動測定

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