Trichodermaセルラーゼ成分のセロオリゴ糖に対する作用様式

概要

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Three purified cellulase components from Meicelase were investigated on K_m values and reaction rates for various normal and reduced cello-oligosaccharides. There were found considerable differences among them, and these differences were roughly in agreement with those found in the mode of action on the hydrolysis of CMC. It seems that cellulases with more random mechanism on the CMC-hydrolysis also revealed a similar tendency in their action on the cello-oligosaccharides. However, all these cellulases had transglycosylation activity, and the results of such kinetic studies were ambiguous. Products of the transglycosylation reaction from normal or reduced cellotetraose (G_4 or G_<4H&gT;) were a mixture of pentasaccharides and higher ones. However, no further investigations were made on these products in the present work. Analysis of bond-attack frequency of three cellulase components toward G_<4H> showed that C III, a cellulase component of 'more random' type on the fydrolysis of CMC, split that sacchride most randomly among the three cellulases, although the frequency of attacking the middle bond was remarkably higher than those for others. C IIand CIV components also showed a preferential attack against the middle bond.
社団法人日本生物工学会の論文
1968-09-25