合成および天然培地におけるTrichoderma virideセルラーゼ成分のカラムクロマトグラフィー分析

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In order to investigate the cause for multiplicity of cellulase of Trichoderma viride, the culture of this fungus on synthetic medium containing Avicel was analyzed for the cellulase components by the similar consecutive column chromatography as used for the commercial products, Meicelase and Cellulase Onozuka, and their resolution patterns were compared with each other. Crude preparation of Cellulase Onozuka was resolved, like Meicelase, into three cellulase peaks, I, II and III by Ameberlite CG-50 column chromatography. The middle fraction, peak II, was further resolved into five peaks, F-I to F-V, by DEAE-Sephadex A-50 column chromatography. However, the enzyme preparations E and L obtained from shake culture on 0.6% Avicel medium contained fewer cellulase component. An insignificant protease activity was detected in E, while a considerably high activity was found in L. Although both E and L gave two cellulase peaks corresponding to peaks II and III of Cellulase Onozuka on Amberlite column chromatography, peak II from E yield F-II alone on the following DEAE-Sephadex column chromatography, but peak II from L produced F-I and F-II. The cellulase inductively formed in washed mycelia by shaking with sophorose showed a similar resolution pattern to that of E on DEAE-Sephadex column chromatography; only a single peak of F-II was found. Moreover, the F-II was converted into F-I in vitro by mixing with a dialyzed filtrate of the glycerol culture, and the presence of the two components after this treatment was validated by DEAE-Sephadex column chromatography.
社団法人日本生物工学会の論文
1968-09-25

合成および天然培地におけるTrichoderma virideセルラーゼ成分のカラムクロマトグラフィー分析

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