放線菌培養液の濾過について (第2報)

概要

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The separation of Streptomyces griseus mycelia from cultured broth by filtration requires a considerable length of time due to a marked resistance of the cake against liquid flow in filtration.An effective method was presented by the authors for the filtering of streptomyces cultured broth at 80-90℃ and pH 3.5-4.0,and, along with a series of experiments, was reported previously in this journal.However, this method cannot be used when heat-unstable substances must be produced from such a broth. Protease production is one such case.In this study, a series of experiments was made to find ways of accelerating the velocity of filtration with a minimum loss of protease activity. When 0.1% (vol./vol.)toluene was added and the temperature of the broth was held at 40℃ and agitated for 3-4 hrs., autolysis occured and easily filtrable broth was obtained with no significant loss of protease activity. With the addition of 10% (wt/vol.) ammonium sulfate prior to filtration, increased velocity of filtration was observed.It was found that the decrease of the insoluble matter was not identical to the increase of filtration velocity.
公益社団法人日本生物工学会の論文
1965-07-25