ラット血清中の副甲状腺ホルモン(PTH)の新ラジオイムノアッセイ系の開発

概要

論文の詳細を見る
Since a suitable antibody has not been found, radioimmunoassay of biologically active parathyroid hormone (PTH) in rat serum has not been well performed. The present paper describes a sensitive radioimmunoassay system for determination of rat PTH using an antiserum with specific recognition sites for the amino-terminal region of rat PTH provided by Dr. Slatopolsky. The assay range of this system was 6.25~100 pg/tube. The CV values of inter- and intra-assay were below 10% which were sufficient. This assay system was applied to serum of normal and vitamin D-deficient rats. a diurnal variation and age difference in the PTH levels was observed, but no significant difference in inter-strains and sexes was found in normal rats. The serum PTH levels in normal rats were significantly lower than those in vitamin D-deficient rats. In conclusion, the higher PTH levels by this assay system responded to the low serum Ca levels and reflected secondary hyperparathyroidism in vitamin D-deficient rats.
日本ビタミン学会の論文
1992-07-25