寄生植物と病原菌との異なる組み合わせによって生成された数種のphytoalexinの紫外線吸収スペクトルの比較

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Experiments were carried out to know if there were any differences among their properties of phytoalexins (PA) produced by different host and parasite combinations. 1) Seed-rooms of opened pea pods were mounted with conidia suspension of each of Ascochytapisi, Glomerella cingulata and Gibberella zeae, and incubated at 20℃ for 24 hours. These three kinds of suspensions were then collected and centrifuged to obtain the supernatants (PA-solution). Five ml of each PA-solution was extracted three times with equal volume of petroleum ether, and then the solvent was evaporated at 80℃. Ethanol was added to each of the residues and ultraviolet absorption of the ethanol solution was measured by spectrophotometer (Shimazu QB-50). Three curves thus obtained showed the same tendency, and was concluded that these three PA substances were chemically the same. 2) Seed-rooms of opened pods of rape (Brassica napus L.) and of the pcds of common vetch (Vicia sativa L.), and leaves of broad bean (Vicia faba L.) from which the epidermis had been peeled off were mounted respectively with conidia suspension of G. cingulata. Thus three PA-solutions of different origins were obtained with the same method as described above. Ten ml of each PA-solution was extracted with equal volume of petroleum ether. Petroleum ether was evaporated under nitrogen gas at 20℃ and 1 ml of 3 per cent ethanol was added to the residue. 0.2 ml of each of three kinds of the liquids was mounted on a hole-slide-glass and its biological activity was assayed on conidia of G. cingulata. Parallel to the above experiment, 0.8 ml of each of the liquids was diluted with 5 ml of ethanol and ultraviolet absorption was measured. Three curves thus obtained and that of the PA liquids from pea were compared and found that these four PAs were distinctly different from each other. It was concluded from these results that chemical nature of PA was specific to the host plant species and was not specific to the fungi that was intrcduced to the hosts.
日本植物病理学会の論文
1964-01-31

寄生植物と病原菌との異なる組み合わせによって生成された数種のphytoalexinの紫外線吸収スペクトルの比較

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