抗生物質の植物体への滲透・吸収・移行 : (その1) 数種抗生物質の検定に及ぼす植物体汁液の影響並びにソラマメによる吸収・移行

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(1) By assaying on a 4-ml agar plate (soluble starch 1%, yeast extract powder 0.1%, agar 1.5%) seeded with a spore suspension of Piricularia oryzae, Antimycin A could be detected at a concentration of 0.01 mcg/ml, Blasticidin at 0.05 mcg/ml and Blastmycin at 0.003 mcg/ml. Antimycin A and Blastmycin were assayed by the paper-disc method and Blasticidin by the cup method. (2) Antimycin A and Blastmycin in plants were extracted by methanol and Blasticidin by the pH 5.0 buffer solution. The juice of leaves of untreated tomato had antibiotic activity against P. oryzae, and conversely that of rice or broad bean promoted its growth. Methanol extracts of leaves of untreated tomato, rice or broad bean gave inhibition zones. Therefore the buffer or methanol extracts of leaves were assayed, after being diluted with buffer or methanol to the concentration in which the plant tissue had no effect upon P. oryzae. (3) Zone sizes of Blasticidin were smaller in broad bean leaf extract than in the buffer solution ; but they were about the same in rice or tomato extract as in the buffer solution. Approximately 80 % of the antifungal activity of Blasticidin decreased in broad bean leaf extract. Zone sizes of Antimycin A and Blastmycin were smaller in rice leaf extract than in methanol, but about the same in tomato or broad bean extract as methanol. Approximately 70% of the antifungal activity of Antimycin A and Blastmycin decreased in rice leaf extract. (4) When the cut stems of broad bean were placed in solutions containing 5〜10 mcg/ml of Antimycin A, Blasticidin and Blastmycin, antibiotics were absorbed and translocated upward to leaves. Leaves were found to contain only 0.1〜0.3mcg/g of Antimycin A or Blastmycin, or 3.0〜6.0 mcg/g of Blasticidin after several days. But no antibiotics were detected in leaves of roottreated plants.
1958-07-30

抗生物質の植物体への滲透・吸収・移行 : (その1) 数種抗生物質の検定に及ぼす植物体汁液の影響並びにソラマメによる吸収・移行

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