抗生物質によるいもち病防除に関する研究 : (その4) Blasticidin S のいもち病菌解糖系コハク酸脱水素酵素, 酸化的燐酸化, 核酸合成および蛋白合成に対する作用

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1) Effect of Blasticidin S on glycolysis in Piricularia oryzae was-examined by measuring lactic acid according to the method of Backer Sammerson, using the suspension of washed mycelium prepared by the method described previously. The glycolysis was almost not inhibited by Blasticidin S, but was remarkably inhibited by phenyl mercuric acetate and monoiodoacetate. 2) Eflect of Blasticidin S on succinic dehydrogenase in cell free extract of the mycelium of Piricularia oryzae was examined by the method in which TTC reduction was measured by the Thunberg technique. Blasticidin S had no significant influence on the succinic dehydrogease. 3) Effect of Blasticidin S on the oxidative phosphorylation by mitochondrial fraction from ratliver was examined. Oxygen consumption was measured manometrically and phosphorylation was estimated by the determination of inorganic phosphate. The oxygen uptake and the oxidative phosphorylation by the rat liver mitochondria were not affected by Blasticidin S, but both reactions were inhibited by phenyl mercuric acetate and Blastmycin. 4) Effect of Blasticidin S on the turnover of inorganic phosphate by respiring mycelium of Piricularia oryzae was studied with the aid of exogenous inorganic phosphate labelled with ^<32>P. ^<32>P from labelled phosphate in the medium was taken up more into the nucleic acid fraction than into the phospholipid or the protein fraction of the mycelium. Each fraction was isolated by the method of Schneider. It was found that the incorporation of ^<32>P into each of the fractions was not influenced by Blasticidin S. 5) Addition of ^<14>C-glutamic acid to the suspension of the mycelium above mentioned resulted in the incorporation of ^<14>C into the mycelium. Since ^<14>C-glutamic acid was incorporated more into the protein fraction than into the nucleic acid or the phospholipid fraction, the incorporation was taken as a measure of examinig protein synthesis. The incorporation into the protein fraction of the mycelium was strongly inhibited by Blasticidin S at a concentration of 1μg/ml. Because this inhibitory concentration was almost equal to the minimum growth inhibitory concentration (0.1-1.0μg/ml), it seemed that the inhibition of protein synthesis was a primary effect of Blasticidin S on the metabolism of Piricularia oryzae.
日本植物病理学会の論文
1961-01-25

抗生物質によるいもち病防除に関する研究 : (その4) Blasticidin S のいもち病菌解糖系コハク酸脱水素酵素, 酸化的燐酸化, 核酸合成および蛋白合成に対する作用

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