腐敗病細菌のペクチン質分解酵素(endo-PTE)による細胞壁多糖質の可溶性化

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Previously, we demonstrated histochemically that pectic substances in middle lamella and primary walls were specifically dissolved by the infection of soft rot pseudomonads. The present investigation was conducted to confirm the characteristics of carbohydrates liberated from cell wall materials during enzymatic maceration, using purified endo-PTE preparation from Pseudomonas marginalis (Brown) Stevens, N-6301 isolate. When radish fiber, instead of cell wall materials, was incubated with the purified enzyme at 30 C in 0.033 M tris-HCl buffer (pH 8.0), a large amount of carbohydrates were released rapidly in water-soluble state at the initial stage of reaction. The total yield of carbohydrates was about 27.5 mg per 100 mg of radish fiber at 90 min. incubation, which corresponds to about 3 to 4 folds of non-enzymatic degradation. About 80% of these water-soluble carbohydrates were galacturonide residue, and about 95% of them were insoluble with 80%-EtOH. It is concluded, therefore, they mainly correspond to pectin-like substances. EtOH-soluble carbohydrates contained two fractions, neutral sugars and oligogalacturonides. In neutral sugar, no visible change was observed between incubation mixture with enzyme and without enzyme. Oligogalacturonides specifically increased by the enzyme action, and the liberation of methyl esters was confirmed by paper chromatographic analysis. These results suggest that oligogalacturonide methyl esters are released from cell wall polysaccharides by trans-eliminative reaction of the enzyme used.
日本植物病理学会の論文
1974-12-25

腐敗病細菌のペクチン質分解酵素(endo-PTE)による細胞壁多糖質の可溶性化

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