Adaptor Polymerase Chain Reaction for Single Molecule Amplification(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
- 社団法人日本生物工学会の論文
- 2005-08-25
著者
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MIZUNO Akira
Department of Astrophysics, Nagoya University
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Mizuno Akira
Dept. Ecological Engineering Toyohashi University Of Technology
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Mizuno A
Dept. Ecological Engineering Toyohashi University Of Technology
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Yasuda Hachiro
Dept. Ecological Engineering, Toyohashi University of Technology
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Katsura Shinji
Dept. of Biological and Chemical Engineering, Gunma University
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NAKANO Michihiko
Department of Ecological Engineering, Toyohashi University of Technology
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KATSURA Shinji
Department of Ecological Engineering, Toyohashi University of Technology
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Komatsu Jun
Department of Ecological Engineering, Toyohashi University of Technology
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Kurita Hirofumi
Department of Ecological Engineering, Toyohashi University of Technology
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Yasuda Hachiro
Department of Ecological Engineering, Toyohashi University of Technology
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Komatsu Jun
Department Of Ecological Engineering Toyohashi University Of Technology
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Katsura Shinji
Dept. Of Biological And Chemical Engineering Gunma University
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Nakano Michihiko
Dept. Ecological Engineering Toyohashi University Of Technology
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Yasuda Hachiro
Dept. Ecological Engineering Toyohashi University Of Technology
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Kurita Hirofumi
Department Of Ecological Engineering Toyohashi University Of Technology
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Mizuno Akira
Department Of Astrophysics Nagoya University
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