Single-Molecule Reverse Transcription Polymerase Chain Reaction Using Water-in-Oil Emulsion(Methods)
スポンサーリンク
概要
- 論文の詳細を見る
A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.
- 社団法人日本生物工学会の論文
- 2005-03-25
著者
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MIZUNO Akira
Department of Astrophysics, Nagoya University
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Mizuno Akira
Dept. Ecological Engineering Toyohashi University Of Technology
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Mizuno A
Dept. Ecological Engineering Toyohashi University Of Technology
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Katsura Shinji
Dept. of Biological and Chemical Engineering, Gunma University
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NAKANO Michihiko
Department of Ecological Engineering, Toyohashi University of Technology
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TAKASHIMA Kazunori
Department of Ecological Engineering, Toyohashi University of Technology
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KATSURA Shinji
Department of Ecological Engineering, Toyohashi University of Technology
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Komatsu Jun
Department of Ecological Engineering, Toyohashi University of Technology
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Kurita Hirofumi
Department of Ecological Engineering, Toyohashi University of Technology
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NAKAI NAOHITO
Department of Ecological Engineering, Toyohashi University of Technology
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Komatsu Jun
Department Of Ecological Engineering Toyohashi University Of Technology
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Nakai Naohito
Department Of Ecological Engineering Toyohashi University Of Technology
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Takashima Kazunori
Department Of Ecological Engineering Toyohashi University Of Technology
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Katsura Shinji
Dept. Of Biological And Chemical Engineering Gunma University
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Nakano Michihiko
Dept. Ecological Engineering Toyohashi University Of Technology
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Kurita Hirofumi
Department Of Ecological Engineering Toyohashi University Of Technology
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Mizuno Akira
Department Of Astrophysics Nagoya University
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