Activation of Restriction Enzyme by Electrochemically Released Magnesium Ion(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Observation and cutting of DNA molecules at intended positions permit several new experimental methods that are completely different from conventional molecular biology methods ; therefore several cutting methods have been proposed and studied. In this paper, a new cutting method for a DNA molecule by localizing the activity of a restriction enzyme is presented. Since most restriction enzymes require magnesium ions for their activation, local restriction enzyme activity can be controlled by the local concentration of magnesium ions. Applying a direct current (dc) voltage to a needle electrode of metallic magnesium made it possible to control the local magnesium ion concentration at the tip of the needle. The restriction enzyme was activated only when magnesium ions were electrochemically supplied.
- 社団法人日本生物工学会の論文
- 2004-10-25
著者
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MIZUNO Akira
Department of Astrophysics, Nagoya University
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Mizuno Akira
Dept. Ecological Engineering Toyohashi University Of Technology
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HARADA Noriaki
Department of Hygiene, Yamaguchi University Graduate School of Medicine
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Maeda Yukihiro
Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University
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Maeda Yukihiro
Department Of Chemistry Faculty Of Science Science University Of Tokyo
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Maeda Yukihiro
Department Of Ecological Engineering Toyohashi University Of Technology
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Mizuno A
Dept. Ecological Engineering Toyohashi University Of Technology
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Katsura Shinji
Dept. of Biological and Chemical Engineering, Gunma University
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TAKASHIMA Kazunori
Department of Ecological Engineering, Toyohashi University of Technology
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KATSURA Shinji
Department of Ecological Engineering, Toyohashi University of Technology
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Komatsu Jun
Department of Ecological Engineering, Toyohashi University of Technology
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MATSUURA SHUN-ICHI
Department of Ecological Engineering, Toyohashi University of Technology
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Komatsu Jun
Department Of Ecological Engineering Toyohashi University Of Technology
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Matsuura Shun-ichi
Department Of Ecological Engineering Toyohashi University Of Technology
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Takashima Kazunori
Department Of Ecological Engineering Toyohashi University Of Technology
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Katsura Shinji
Dept. Of Biological And Chemical Engineering Gunma University
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Harada Noriaki
Department Of Ecological Engineering Toyohashi University Of Technology
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Harada Noriaki
Department Fo Hygiene Yamaguchi University School Of Medicine
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Mizuno Akira
Department Of Astrophysics Nagoya University
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