Isolation, Characterization, and Antitumor Activities of the Cell Wall Polysaccharides from Elsinoe leucospila

概要

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A cell-wall preparation from the cells of Elsinoe leucospila, which produces elsinan extracellularly when grown on sucrose or glucose-potato extract medium, was fractionated systematically. The heteropolysaccharide that was released by treatment with Actinase E digestion, comprised D-mannose, D-galactose, and D-glucose (molar ratio, 1.5 : 1.0 : 0.1). Methylation, mild acid hydrolysis, and ^13C-NMR studies suggested that the polysaccharide contains a backbone of α-(1→6)-linded D-mannose residues having two kinds of side chains, one attached at the O-4 with single or short β-(1→6)-linked D-galactofuranosyl residues, and the other attached at O-2 with short side chains, most probably, of α-(1→3)-linked D-mannopyranosyl residues. A moderately branched D-glucan fraction, obtained from the cold alkali extract, was fractionated to give an antitumor-active purified β-(1→3)-glucan having branches of single β-D-glucosyl groups, one out of eight D-glucose residues being substituted at the O-6.
社団法人日本農芸化学会の論文
1992-01-23