Benzene Toxicity and Yield Improvement in a cis-Benzeneglycol Production Process

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概要

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• We carried out experiments designed to increase the production of cis-benzeneglycol from benzene by a mutant strain of Pseudomonas. Factors affecting the production were the temperature, the nitrogen source and it's concentration, the concentration of iron ions, and the benzene concentration. At a benzene concentration of 33% of saturation vapor pressure, the cis-benzeneglycol production was not stable, but this benzene concentration was not toxic to the cells. In a culture under 10% of saturation vapor pressure the cells kept producing cis-benzeneglycol for over 100 h.The decrease in cis-benzeneglycol production observed at high benzene partial pressure (33%) could not be accounted for by the combined effects of an inhibition of enzyme production and a rapid turn-over of the enzyme, but by a specific inactivation of the enzyme. Possible mechanisms of inactivation were discussed which could explain the specific inactivation of the benzene dioxygenase : generation of polyphenols or generation of oxygen radicals.

• 社団法人日本生物工学会の論文
• 1988-06-25

著者

• Kawakami Yasushi

  Department Of Chemical Engineering University Of Tokyo

• YARMOFF JEAN-JACQUES

  Department of Chemical Engineering, University of Tokyo

• YAGO TAKESHI

  Department of Chemical Engineering, University of Tokyo

• NISHIMURA HAJIME

  Department of Chemical Engineering, University of Tokyo

• Kawakami Yasushi

  Department Of Biology Faculty Of Education Tottori University:(present Address)tottori West Technica

• Yago Takeshi

  Department Of Chemical Engineering University Of Tokyo

• Kawakami Yasushi

  (present Address)institute Of Research And Innovation Biotechnology Department

• NISHIMURA HISATAKA

  Department of Chemical and Biochemical Engineering, Toyama National College of Technology

• Yarmoff Jean-jacques

  Department Of Chemical Engineering University Of Tokyo

• Nishimura Hisataka

  Department Of Chemical And Biochemical Engineering Toyama National College Of Technology

• Nishimura Hajime

  Department Of Chemical Engineering Faculty Of Engineering University Of Tokyo

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