Improvement of Xylitol Production by Controlling Oxygen Supply in Candida parapsilosis

概要

論文の詳細を見る
Fermentor experiments were carried in which the oxygen transfer coefficient (k_La)was varied in order to examine quantitatively the effect of oxygen on xylitol production. When the k_La was increased from 20 to 85 h^<-1>, the cell cencentration increased. However, the maximum production of xylitol from 50 g/l xylose, 35.8 g/l, was achieved at 62 h at k_La of 30 h^<-1>. Cultures for xylitol production were also carried out under controlled DO concentrations. The cell concentration and xylitol dehydrogenase activity increased with increasing the DO concentration. At a high level of xylitol dehydrogenase activity, most of the xylitol was converted into xylulose, which was metabolized to cell material. The maximum xylitol production from 50 g/l xylose, 36 g/l, was obtained after 54 h and the activity of xylose reductase was maximum at a DO concentration of 0.7%, indicating that the DO concentration must be carefully controlled for efficient xylitol production from xylose. By controlling the DO concentration in the range from 0.8 to 1.2%, a final xylitol concentration of 210 g/l which corresponded to a xylitol yield of 70%, was obtained from 300 g/l xylose in 66 h.
社団法人日本生物工学会の論文
1997-03-25