Production of 8-Hydroxydaidzein from Soybean Extract by Means of Aspergillus oryzae KACC 40247
スポンサーリンク
概要
- 論文の詳細を見る
Aspergillus oryzae KACC 40247 was selected from among 60 fungal strains as an effective 7,8,4'-trihydroxyisoflavone (8-hydroxydaidzein)-producing fungus. The optimal culture conditions for production by this strain in a 7-L fermentor were found to be 30 °C, pH 6, and 300 rpm. Under these conditions, A. oryzae KACC 40247 produced 62 mg/L of 8-hydroxydaidzein from soybean extract in 30 h, with a productivity of 2.1 mg/L/h. These are the highest production and productivity for 8-hydroxydaidzein ever reported. To increase production, several concentrations of daidzin and of daidzein as precursor were added at several culture times. The optimal addition time and concentration for daidzin were 12 h and 1,248 mg/L, and those for daidzein were 12 h and 254 mg/L respectively. Maximum production and productivity for 8-hydroxydaidzein with the addition of daidzein were 89 mg/L and 2.9 mg/L/h respectively, and those with the addition of daidzin were 160 mg/L and 4.4 mg/L/h respectively.
著者
-
Oh Deok-Kun
Department of Bioscience and Biotechnology, Konkuk University
-
Oh Deok-kun
Department Of Bioscience And Biotechnology Konkuk University
-
Kim Kyoung-rok
Department Of Bioscience And Biotechnology Konkuk University
-
SEO Min-Ho
Department of Bioscience and Biotechnology, Konkuk University
-
KIM Bi-Na
Department of Bioscience and Biotechnology, Konkuk University
-
LEE Ki
WCU Biomodulation Major, Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University
-
LEE Choong-Hwan
Department of Bioscience and Biotechnology, Konkuk University
関連論文
- Substrate specificity of a recombinant D-lyxose isomerase from Providencia stuartii for monosaccharides(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Characterization of a recombinant β-glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Biological Modification of the Fatty Acid Group in an Emulsan by Supplementing Fatty Acids under Conditions Inhibiting Fatty Acid Biosynthesis
- Ginsenoside Compound K Production from Ginseng Root Extract by a Thermostable β-Glycosidase from Sulfolobus solfataricus
- Biological Modification of Hydrophobic Group in Acinetobater calcoaceticus RAG-1 Emulsan
- Improvement of Xylitol Production by Controlling Oxygen Supply in Candida parapsilosis
- Production of the Rare Ginsenosides Compound K, Compound Y, and Compound Mc by a Thermostable β-Glycosidase from Sulfolobus acidocaldarius
- Ginsenoside Compound K Production from Ginseng Root Extract by a Thermostable β-Glycosidase from Sulfolobus solfataricus
- L-Arabinose production from sugar beet arabinan by immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus in a packed-bed reactor(BIOCHEMICAL ENGINEERING)
- Enhancement of retinal production by supplementing the surfactant Span 80 using metabolically engineered Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Enhancement of retinal production by supplementing the surfactant Span 80 using metabolically engineered Escherichia coli
- Production of 8-Hydroxydaidzein from Soybean Extract by Means of Aspergillus oryzae KACC 40247