Enhancement of retinal production by supplementing the surfactant Span 80 using metabolically engineered Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• The optimal temperature and pH for retinal production using metabolically engineered Escherichia coil in a 7-1 fermentor were found to be 30℃ and 7.0, respectively. The agitation speed was a critical factor for retinal production. The optimal agitation speed was 400 rpm (oxygen transfer coefficient, k_La, = 92 1/h) in batch culture and 600 rpm (k_La = 148 1/h) in a fed-batch culture of glycerol Span 80 was selected as a surfactant for retinal production in metabolically engineered E. coil because Span 80 had proven the most effective for increased retinal production among the tested surfactants. Under the optimal conditions in the fed-batch culture with 5 g/l Span 80, the cell mass and the concentration, content, and productivity of retinal were 24.7 g/l, 600 mg/l, 24.3 mg/g-cells, and 18 mgl^<-1> h^<->1 after 33 h, respectively. They were 1.2-, 2.7-, 2.3-, and 2.7-fold higher than those in the fed-batch culture without Span 80, respectively. The concentration and productivity of retinal in this study were the highest ever reported. The hydrophilic portion of Span 80 (sorbitan) did not affect cell growth and retinal production, but the hydrophobic portion (oleic acid) stimulated cell growth. However, oleic acid plus sorbitan did not stimulate retinal production. Thus, Span 80, as a linked compound of oleic acid and sorbitan produced by esterification, proved to be an effective surfactant for the enhancement of retinal production.

• 公益社団法人日本生物工学会の論文

著者

• Oh Deok-kun

  Department Of Bioscience And Biotechnology Konkuk University

• Kim Yeong-su

  Department Of Bioscience And Biotechnology Konkuk University

• Kim Seon-won

  Division Of Applied Life Science (bk21) Eb-ncrc And Pmbbrc Gyeongsang National University

• Lee Jae-hee

  Department of Bioscience and Biotechnology, Konkuk University

• Choi Jin-Geun

  Department of Bioscience and Biotechnology, Konkuk University

• Kim Kyoung-Rok

  Department of Bioscience and Biotechnology, Konkuk University

• Lee Jae-hee

  Department Of Bioscience And Biotechnology Konkuk University

• Choi Jin-geun

  Department Of Bioscience And Biotechnology Konkuk University

• Kim Kyoung-rok

  Department Of Bioscience And Biotechnology Konkuk University

関連論文

• Substrate specificity of a recombinant D-lyxose isomerase from Providencia stuartii for monosaccharides(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Characterization of a recombinant β-glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Biological Modification of the Fatty Acid Group in an Emulsan by Supplementing Fatty Acids under Conditions Inhibiting Fatty Acid Biosynthesis
• Biological Modification of Hydrophobic Group in Acinetobater calcoaceticus RAG-1 Emulsan
• Improvement of Xylitol Production by Controlling Oxygen Supply in Candida parapsilosis
• FM-P4 Beta-carotene production of recombinant Escherichia coli engineered with whole mevalonate pathway or non-mevalonate pathway in batch cultures at different temperatures and pH(Section VIII Fermentation and Microbial Technology)
• Production of the Rare Ginsenosides Compound K, Compound Y, and Compound Mc by a Thermostable β-Glycosidase from Sulfolobus acidocaldarius
• Ginsenoside Compound K Production from Ginseng Root Extract by a Thermostable β-Glycosidase from Sulfolobus solfataricus
• L-Arabinose production from sugar beet arabinan by immobilized endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus in a packed-bed reactor(BIOCHEMICAL ENGINEERING)
• Enhancement of retinal production by supplementing the surfactant Span 80 using metabolically engineered Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Enhancement of retinal production by supplementing the surfactant Span 80 using metabolically engineered Escherichia coli
• Enhancement of geraniol resistance of Escherichia coli by MarA overexpression(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production of 8-Hydroxydaidzein from Soybean Extract by Means of Aspergillus oryzae KACC 40247

もっと見る 閉じる

スポンサーリンク