Improvement of Coenzyme Q_<10> Production by Increasing the NADH/NAD^+ Ratio in Agrobacterium tumefaciens

概要

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Previously screened CoQ10-overproducing Agrobacterium tumefaciens A603-35 showed a relatively high NADH/NAD+ ratio (1.1), as compared to parental strain C58 (0.2) when we increased the expression levels of NADH-generating enzymes. Also, the intracellular NADH/NAD+ ratio showed a positive correlation with the CoQ10 content in A603-35. Overexpression of glyceraldehyde 3-phosphate dehydrogenase in A603-35 shifted the NADH/NAD+ ratio at 48 h from 0.8 to 1.2, and thus the CoQ10 content in flask culture increased from 2.16 to 3.63 mg/g DCW. Due to the addition of hydroxybutyrate to the culture media, the intracellular NADH/NAD+ ratio in A603-35-gapA shifted from 1.2 to 1.4, which led to an increase CoQ10 content (5.27 mg/g DCW).
社団法人日本農芸化学会の論文
2010-04-23