Biological Oxidation of Alkane to Alkene under Anaerobic Conditions

概要

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A petroleum assimilating facultative anaerobic bacterium (strain HD-1) was isolated from an oil field in Shizuoka. The cells were anaerobically grown on CO_2 as the sole carbon source in the presence of H_2,and the growth was markedly enhanced by the addition of aromatic or aliphatic hydrocarbon [Morikawa, M. and Imanaka, T. : J. Ferment. Bioeng., 76,280-283,1993]. The strain degraded tetradecane (C14) under anaerobic conditions and one of the major metabolic intermediates was identified as 1-dodecene (C12). This result demonstrates that alkanes are biodegradable in the absence of molecular oxygen via a pathway different from that of β-oxidation. The strain was found to grow on CO_2 in the presence of tetradecane and absence of H_2 gas and light. These findings strongly indicate that the bacterium is capable of anaerobically utilizing alkane as an energy source.
公益社団法人日本生物工学会の論文
1996-09-25