Development of Two Stage Culture Process by Optimization of Inorganic Salts for Improving Catharanthine Production in Hairy Root Cultures of Catharanthus roseus
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概要
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The effects of the concentration of inorganic salts in Schenk and Hildebrandt (SH) medium on catharanthine production in hairy root cultures of Catharanthus roseus were investigated. The inorganic salt components could be categorized into four groups. The first group (nitrate) supported both the growth of and catharanthine production by hairy roots with incremental increases in the concentration. The second (ammonium and phosphate) yielded contradictory effect with respect to growth and production. The third (borate and molibdate) inhibited both growth and production, while the fourth (potassium iodide, sulfate, and iron) did not exhibit any significant effects. Through optimization of the concentrations of inorganic salts in the medium, a two stage process of hairy root cultures with different media for growth and production was developed which enabled us to enhance the volumetric yield of catharanthine up to 60.5mg/l. This productivity was 5.4 times higher than that of a one stage culture in the original SH medium.
- 社団法人日本生物工学会の論文
- 1994-01-25
著者
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Liu Jang
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology
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Jung K‐h
Korea Res. Inst. Biosci. And Biotecnol. Taejon Kor
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Kwak Sang-soo
Department Of Agricultural Chemistry The University Of Tokyo:(present)genetic Engineering Center Kor
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Choi C‐y
Seoul National Univ. Seoul Kor
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Liu Jang
Plant And Animal Cell Technology Research Division Korea Research Institute Of Bioscience
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Liu Jang
Plant Cell Biology Laboratory Genetic Engineering Research Institute Korea Institute Of Science And
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Kwak S‐s
Korea Res. Inst. Of Bioscience And Biotechnology Taejon Kor
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Kwak Sang-soo
Plant And Animal Cell Technology Research Division Korea Research Institute Of Bioscience
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JUNG KYUNG-HEE
Plant Cell Biology Laboratory, Genetic Engineering Research Institute, Korea Institute of Science an
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CHOI CHA-YONG
Biochemical Engineering Major School of Engieering, Seoul National University
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Jung Kyung-hee
Plant And Animal Cell Technology Research Division Korea Research Institute Of Bioscience
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Kwak Sang-soo
Plant Cell Biology Laboratory Genetic Engineering Research Institute Korea Institute Of Science And
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