Enhanced Production of Shikonin by Lithospermum erythrorhizon Cells Immobilized in Polyurethane Foam Matrices

概要

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Production of shikonin by Lithospermum erythrorhizon cells immobilized in polyurethane foam matrices was investigated. The cells were effectively entrapped inside the polymer matrices prepared in a cubic form with a lateral length of 0.5 cm. They proliferated further by incubation and resulted in immobilized polymer foam cubes which were fully packed with cell aggregates. Shikonin production was observed to increase significantly when the immobilized cells were used. It was excreted by the cells and directly adsorbed, by hydrophobic interaction, to the polymer site. The product could be recovered, after the cells were separated, by direct solvent extraction from the shikonin-adsorbed polyurethane matrices. It is more interesting to note that a single-stage culture with immobilized L. erythrorhizon cells yielded higher shikonin productivity than the conventional two-stage culture system, which is considered to be of practical importance for industrial applications.
公益社団法人日本生物工学会の論文
1990-11-25