Pollen-Baiting and Drying Method for the Highly Selective Isolation of Actinoplanes spp. from Soil

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概要

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• A simplified enrichment method for the highly selective isolation of the zoosporic actinomycetes Actinoplanes spp. from soil is described. The method consists of baiting the species with Pinus pollen grains, desiccating (30℃, 2 h) the baits bearing sporangia in dried soil particles with the aid of silica gel and following the spore liberation upon immersion in water. Portions of the liquid enrivhed with zoospores are plated out on humic acid-vitamin (HV) agar supplemented with nalidixic acid at a concentration of 10 μg ml^<-1>. The desiccation stage has enabled the almost complete elimination of associated bacteria from colonized baits while allowing the Actinoplanes sporangia to survive and still posses the ability to release many spores. A total of four different soil samples from fields of corn, peach, vegetable and paddy rice were examined. The pollen-baiting and drying method consistently resulted in the highly selective isolation of Actinoplanes spp. which accounted for over 83% of the total number of micro-organisms recovered on HV agar containing nalidixic acid.

• 社団法人日本生物工学会の論文
• 1991-12-25

著者

• Tamura Tomohiko

  Nite Biological Resource Center (nbrc) National Institute Of Technology And Evaluation

• Hayakawa Masayuki

  Department Of Fermentation Technology Faculty Of Engineering Yamanashi University

• Hayakawa Masayuki

  Department Of Applied Chemistry And Biotechnology

• Hayakawa M

  Division Of Applied Biological Sciences Interdisciplinary Graduate School Of Medicine And Engineerin

• Hayakawa Masayuki

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• NONOMURA HIDEO

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• TAMURA TOMOHIKO

  (Present address)Institute for Fermentation

• IINO HIROMITSU

  Department of Fermentation Technology, Faculty of Engineering, Yamanashi University

• Nonomura H

  Department Of Applied Chemistry And Biotechnology Yamanashi University

• Nonomura Hideo

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Iino H

  Yamanashi Univ. Kofu Jpn

• Iino Hiromitsu

  Department Of Applied Chemistry And Biotechnology

• Hayakawa Masayuki

  Interdisciplinary Graduate School Of Medicine And Engineering University Of Yamanashi

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