Efficacy of Artificial Humic Acid as a Selective Nutrient in HV Agar Used for the Isolation of Soil Actinomycetes

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概要

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• The efficacies of various kinds of humic acid, as the sole source of carbon and nitrogen in HV agar reported in the previous paper, were compared to the selective isolation of soil actinomycetes. The 4 types of natural humic acid, A, B, P and Rp were prepared from different soils, and 3 kinds of artificial humic acid were made from carbohydrates and urea in our laboratory.Among the natural humic acids, type Rp, which has been reported to be associated with an initial stage of humification in natural conditions, showed the greatest efficacy.However, one of the artificial humic acids, which was prepared from glucose and urea, was considered to be superior to the Rp natural humic acid : 1) The HV agar containing this artificial humic acid (HV-glucose HA agar) produced the same large number of actinomycete colonies on the plate as that of the HV agar with type Rp soil humic acid (HV-Rp agar). 2) The HV-glucose HA agar restricted the number of bacterial colonies on the plates to one-half of that on HV-Rp agar plates. 3) The quality of natural humic acids varies, whereas artificial ones are more constant and can be made in any laboratory.

• 社団法人日本生物工学会の論文
• 1987-12-25

著者

• Hayakawa Masayuki

  Department Of Fermentation Technology Faculty Of Engineering Yamanashi University

• Hayakawa Masayuki

  Department Of Applied Chemistry And Biotechnology

• NONOMURA HIDEO

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• Nonomura Hideo

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

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• Chemotaxis in the Zoosporic Actinomycete Catenuloplanes japonicus
• New Methods for the Highly Selevtive Isolation of Streptosporangium and Dacrylosporangium from Soil
• New Methods for the Highly Selective Isolation of Micromonospora and Microbispora from Soil
• Humic Acid-Vitamin Agar, a New Medium for the Selective Isolation of Soil Actinomycetes
• Distribution of Rare Actinomycetes in Japanese Soils
• Efficacy of Artificial Humic Acid as a Selective Nutrient in HV Agar Used for the Isolation of Soil Actinomycetes
• Combined Use of Trimethoprim with Nalidixic Acid for the Selective Isolation and Enumeration of Actinomycetes from Soil
• Application of a Method Incorporating Treatment with Chloramine-T for the Selective Isolation of Streptosporangiaceae from Soil
• Inherent G418-Resistance in Hybridization of Industrial Yeasts
• Production of C_2-Symmetrical Phenazines by Some Actiomycetes
• Use of Chloramphenicol-Inherent Resistance in Protoplast Fusion of Industrial Yeast Strains
• Distribution of Actinoplanetes in Soils of Japan

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