New Methods for the Highly Selective Isolation of Micromonospora and Microbispora from Soil

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概要

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• Two plate culture methods for the highly selective isolation of the soil actinomycetes Micromonospora and Microbispora, are described, which utilize the differential antibiotic resistance of these microbes and the superior ability of their spores to withstand deleterious agents. (i) To isolate micromonosporae, a water suspension of air-dried soil is first treated with 1.5% phenol, diluted with water and then cultured on HV agar supplemented with both tunicamycin and nalidixic acid. (ii) To isolate microbisporae, ait-dried soil is heated in a hot-air oven at 120℃ for 1 h. A water suspension of the heated sample is then treated with a solution of 1.5% phenol and 0.03% chlorhexidine gluconate (CG), diluted and cultured on HV agar supplemented with nalidixic acid. The phenol pretreatment of the soil killed bacteria and streptomycetes in the samples, while keeping micromonosporae and microbisporae alive. The use of tunicamycin suppressed the growth of the remaining microbisporae on HV agar isolation plates, thereby facilitating the intensive isolation of micromonocporae. On the other hand, the dry heat treatment of the soil drastically reduced the number of bacteria in the sample and significantly reduced the numbers of streptomycetis and micromonosporae, without reducing the microbisporae content. Further treatment with a mixture of phenol and CG eliminated dry heat-resisting actinomycetes apart from microbisporae. Dilution plating of samples subjected to heat and the phenol-CG treatment resulted in the intensive isolation of microbisporae on HV agar. From various field soils (vegetable, orchard, corn, and rice paddy), the method described above always enabled the highly selective isolation of micromonosporae and microbisporae, respectively, as each group accpimted for more than 90% of the total number of colonies recovered.

• 社団法人日本生物工学会の論文
• 1991-11-25

著者

• kajiura takayuki

  Department of Cardiovascular Center, Biwakoohashi Hospital

• Hayakawa Masayuki

  Department Of Fermentation Technology Faculty Of Engineering Yamanashi University

• Hayakawa Masayuki

  Department Of Applied Chemistry And Biotechnology

• Hayakawa M

  Division Of Applied Biological Sciences Interdisciplinary Graduate School Of Medicine And Engineerin

• Kajiura Takayuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University:(prese

• Hayakawa Masayuki

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• NONOMURA HIDEO

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• Nonomura H

  Department Of Applied Chemistry And Biotechnology Yamanashi University

• Nonomura Hideo

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• SADAKATA TETSUO

  Department of Fermentation Techonology, Faculty of Engineering, Yamanashi University

• Sadakata Tetsuo

  Department Of Fermentation Techonology Faculty Of Engineering Yamanashi University:(present Address)

• Hayakawa Masayuki

  Interdisciplinary Graduate School Of Medicine And Engineering University Of Yamanashi

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