Use of Chloramphenicol-Inherent Resistance in Protoplast Fusion of Industrial Yeast Strains

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概要

• 論文の詳細を見る

• Thirty strains, consisting of industrial yeast strains, e. g. wine yeast Saccharomyces cerevisiae W3 etc., were screened for inherent resistance to 0.5% chloramphenicol (CM). Ten (33%) of the 30 strains showed inherent resistance to the inhibitory effect of 0.5% CM. The petites converted from the CM-inherent resistants by treating with acriflavine or ethidium bromide retained the CM-resistance (ρ^- CMR), and thus were fused by the Ca^<2+>PEG method with industrial strains (ρ^+ CMS). The fusion products were selected in glycerol medium with 0.5% CM and isolated at frequencies ranging from 1.4×10^<-6> to 3.3×10^<-6> for used protoplasts. In this case, the reversion rates were less than 10^<-9>. The resulting fusion products were CM-resistant strains showing respiratory competence (ρ^+ CMR). Thus it was dumonstrated that the fusion products contained the traits (ρ^+ and CMR) of both parents. The CM-inherent resistance of industrial yeast strains may be used as a suitable selection marker in the protoplast fusion of industrial strains, for which it is preferable to avoid mutagenesis.

• 公益社団法人日本生物工学会の論文
• 1991-10-25

著者

• Nonomura Hideo

  Department Of Fermentation Technology Faculty Of Engineering Yamanashi University

• Nonomura Hideo

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Yamazaki T

  Department Of Applied Chemistry And Biotechnology Yamanashi University

• Yamazaki Toyohiko

  Department Of Applied Chemistry And Biotechnology

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