Inherent G418-Resistance in Hybridization of Industrial Yeasts
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概要
- 論文の詳細を見る
Eight strains of sake yeast exhibited inherent-resistnace to 100 μg/ml of Geneticin (G418). Fourteen wine yeasts and 1 shochu yeast (Saccharomyces cerevisiae) and 1 miso yeast (Zygosaccharomyces rouxii) were inherent G418-sensitive. The petites converted from inherent G418-resistants by treatment with ethidium bromide retained G418-resistance (ρ^- G418R), and thus were hybridized by electrofusion with the wine yeast W3(ρ^+ G418S, wild type). A lag phase of 12-18 h was required prior to administration of the drug in glycerol medium when selecting G4180-resistant hybridization products. Colonies were formed in the regeneration medium at a frequency of about 1×10^<-5> per used protoplasts. No growth of any parental strain (10^6〜10^7 protoplasts) separately subjected to electrofusion and regeneration was observed. The hybridization products were G418-resistant"grande"strains (ρ^+ G418R) in which the genetic traits of parental strains had been complemented. Uninucleate cells (DAPI staining) of the hybridization products showed CHEF electrophoretic karyotypes similar to that of wine yeast, but possessed a single chromosome (approx. 320 kb) presumably from sake yeast.
- 社団法人日本生物工学会の論文
- 1994-02-25
著者
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YAMAZAKI Toyohiko
Department of Applied Chemistry and Biotechnology, Yamanashi University
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NONOMURA HIDEO
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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Nonomura Hideo
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Yamazaki Toyohiko
Department Of Applied Chemistry And Biotechnology
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Yamazaki Toyohiko
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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