Humic Acid-Vitamin Agar, a New Medium for the Selective Isolation of Soil Actinomycetes

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概要

• 論文の詳細を見る

• A new medium, designated HV agar, containing soil humic acid as the sole source of carbon and nitrogen was developed.The HV agar was superior to other currently used media, including colloidal chitin agar, glycerol-arginine agar and starch-casein-nitrate agar, for the isolation and enumeration of soil actinomycetes : It allowed the growth of the largest numbers of actinomycete colonies belonging to each genus of Streptomyces, Micromonospora, Microbispora, Steptosporangium, Nocardia, Dactylosporangium, Microtetraspora and Thermomonospora on the plate, while restricting the development of true bacteria. The HV agar supported adequate growth and good sporulation for these actinomycetes.Even when spore suspensions were used as the inoculum, the HV agar produced remarkably larger numbers of actinomycetes, especially strains of the genera Micromonosphora, Microbispora, Steptosporangium, Dactylosporangium and Saccharomonospora, than did glycerol-arginine agar. It was foun that the spores of these actinomycetes were activated upon germination by treatment at 20℃ for 30 min with a 0.2% solution of humic acid prior to incubation.

• 社団法人日本生物工学会の論文
• 1987-10-25

著者

• Hayakawa Masayuki

  Department Of Fermentation Technology Faculty Of Engineering Yamanashi University

• Hayakawa Masayuki

  Department Of Applied Chemistry And Biotechnology

• Hayakawa M

  Division Of Applied Biological Sciences Interdisciplinary Graduate School Of Medicine And Engineerin

• Hayakawa Masayuki

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• NONOMURA HIDEO

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• Nonomura H

  Department Of Applied Chemistry And Biotechnology Yamanashi University

• Nonomura Hideo

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Hayakawa Masayuki

  Interdisciplinary Graduate School Of Medicine And Engineering University Of Yamanashi

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