Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading
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概要
- 論文の詳細を見る
Primary rat hepatocytes form discontinuous monolayers even at their maximum density. Here, we show that staurosporine promotes attachment and spreading of hepatocytes onto culture substrates, so that hepatocytes form a close, continuous monolayer. This treatment did not attenuate major hepatic functions. Therefore, this technique is promising for making seamless cell-sheet structures, which will be applicable for cell-polarity experiments or artificial liver construction.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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SAITO Takao
Department of Materials Chemistry , Graduate School of Osaka University
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MAEDA Sumio
Department of Technology, Kagoshima University
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Lin Konghua
Department Of Chemistry National Industrial Research Institute Of Nagoya
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Maeda S
Kyoto Univ. Kyoto Jpn
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INAGAKI Hidetoshi
Department of Chemistry, National Industrial Research Institute of Nagoya
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LIN Kong-Hua
Department of Chemistry, National Industrial Research Institute of Nagoya
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Maeda Sumio
Department Of Chemistry National Industrial Research Institute Of Nagoya
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Inagaki Hidetoshi
Department Of Chemistry National Industrial Research Institute Of Nagoya
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Saito Takao
Department Of Gastroenterological Surgery National Kyushu Cancer Center
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Saito Takao
Department Of Biochemistry And Surgery Oita Medical School
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MAEDA Sumio
Department of Agricultural Chemistry, The University of Tokyo
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