Long-term Culture of Primary Rat Hepatocytes on Heparin- or Lambda Carrageenan-containing Collagen Gels

スポンサーリンク

概要

• 論文の詳細を見る

• The interactions of glycosaminoglycans (GAGs) with collagen are thought to be important in cell adhesion and cell differentiation. To investigate whether the interactions of GAG or sulfated polysaccharide with collagen can maintain the functions of cultured primary rat hepatocytes, GAG- or sulfated polysaccharide-containing collagen gels were reconstituted in vitro and used for culture of hepatocytes. Among the GAGs and sulfated polysaccharides examined, heparin- and lambda carrageenan-containing collagen gels were found to be able to stimulate and sustain albumin synthesis, while the other GAG- or sulfated polysaccharide-containing collagen gels had almost no effect on maintenance of albumin synthesis. In the cultures using collagen gels that contained 400 μg/ml heparin or 100 μg/ml lambda carrageenan, albumin synthesis by rat hepatocytes was prolonged to about 4 and 5 weeks, respectively, but albumin synthesis was kept up for only one week in the cultures using conventional collagen gels. These results suggest that the interactions of heparin or lambda carrageenan with collagen might be of importance for long-term maintenance of hepatocyte functions.

• 社団法人日本農芸化学会の論文
• 1997-06-23

著者

• SAITO Takao

  Department of Materials Chemistry , Graduate School of Osaka University

• MAEDA Sumio

  Department of Technology, Kagoshima University

• Lin Konghua

  Department Of Chemistry National Industrial Research Institute Of Nagoya

• Maeda S

  Kyoto Univ. Kyoto Jpn

• INAGAKI Hidetoshi

  Department of Chemistry, National Industrial Research Institute of Nagoya

• LIN Kong

  Biotechnology Department, Institute of Research and Innovation

• Maeda Sumio

  Department Of Chemistry National Industrial Research Institute Of Nagoya

• Inagaki Hidetoshi

  Department Of Chemistry National Industrial Research Institute Of Nagoya

• Saito Takao

  Department Of Gastroenterological Surgery National Kyushu Cancer Center

• Saito Takao

  Department Of Biochemistry And Surgery Oita Medical School

• MAEDA Sumio

  Department of Agricultural Chemistry, The University of Tokyo

関連論文

• Crystal Structure of Long Alkyl 3-(Thymin-1-yl)propionates: Style of Hydrogen Bonding and Dependence on the Alkyl Chain Length
• Prognostic Significance of Loss of Heterozygosity on Chromosome 9p21-22 in Human Esophageal Cancer
• Expression of a Thermostable Cellulase Gene from a Thermophilic Anaerobe in Saccharomyces cerevisiae
• Optimization of the Expression of a Foreign Gene from Galactose-Inducible Promoter in a Recombinant Yeast
• Increased Production of Cloned β-Galactosidase in Two-Stage Culture of Bacillus amyloliquefaciens
• BASIC STUDY ON MEMBRANE-ACTIVE AGENTS THAT POTENTIATE ANTICANCER ANTIBIOTICS
• The harmonic characteristics of 40W-fluorescent lamp operating circuit with a leakage transformer
• Reversible Photodimerization of Ester Derivatives of Thymine Having Long Alkyl Chains in Solid Film
• Overproduction of Thermostable β-Galactosidase in Escherichia coli, Its Purification and Molecular Structure
• Eficient Preparation of Optically Active Ketoprofen by Mucor javanicus Lipase Immobilized on an Inorganic Support
• Nucleotide Sequence of the LacN Gene Encoding Thermostable β-Galactosidase of a Thermophilic Anaerobe, Strain NA10
• Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading
• Long-term Culture of Primary Rat Hepatocytes on Heparin- or Lambda Carrageenan-containing Collagen Gels
• DNA Fragmentation Induced in High-Cell-Density Culture of Primary Rat Hepatocytes In an Active Process Dependent on Energy Availability, Gene Expression, and Calmdulin
• Inhibition by Retinoic Acid of Albumin and DNA Synthesis in Adult Rat Hepatocytes
• Inhibition by Apple Polyphenols of ADP-Ribosyltransferase Activity of Cholera Toxin and Toxin-Induced Fluid Accumulation in Mice
• Appendiceal Intussusception Due to an Appendiceal Malignant Polyp-An Association in a Patient with Peutz-Jeghers Syndrome : Report of a Case
• Malignant Hypertension and Microangiopathic Hemolytic Anemia
• A Modified Colorimetric MTT Assay Adapted for Primary Cultured Hepatocytes : Application to Proliferation and Cytotoxicity Assays
• Preparation and Reactions of 1,2-Thiaphospholes
• The Swelling of the External Ear in an Old Woman
• ENTRAPMENT NEUROPATHY OF RADIAL NERVE : A REVIEW OF 4 CASES
• Significance of fat stains in serial sections from Epon-embedded tissue samples for electron microscopy in renal diseases
• 1,8-Diazabicyclo[5.4.0]undec-7-ene-promoted Regioselective Elimination of Vicinal Dibromides Having an Adjacent O- and/or N-Functional Group
• Chemistry of 1,2-thiaphosphole 2-sulfides. II. Reactions of 1,2-thiaphosphole 2-sulfides with some nucleophiles.
• Chemistry of 1,2-thiaphosphole 2-sulfides. I. Cycloaddition reactions of 1,2-thiaphosphole 2-sulfides with some dienophiles.
• Crystal and molecular structure of 3,8-di-t-butyl-5,6-diphenyl-1-thioxo-2,9-dithia-1-phosphabicyclo(4.3.0)nona-3,7-diene.
• Crystal and molecular structure of 4-t-butyl-5-cyano-2-phenyl-1-thioxo-7-thia-1-phosphabicyclo(2.2.1)hept-2-ene.
• Reactions of .ALPHA.,.BETA.-unsaturated thiones with diphenylcyclopropenone and diphenylcyclopropenethione.
• The reaction of fluorenethione S-tosylimide with Grignard reagents.
• Thione S-imide. Reaction with heterocumulenes.
• The Reaction of Isocyanates with Carboxylic Acids and Thiocarboxylic Acids
• Thione S-imides. The reaction with carbon-hetero atom double bond.
• The Wittig-type reaction of O=C-, C=C-, and N=C-substituted methylenetriphenylphosphoranes with N-sulfinyl-p-toluenesulfonamide. An intramolecular 1,3-dipolar cyclization of imidoyl-conjugated thione S-imides.
• Purification and Characterization of a Surface-active Macropeptide Produced from Succinylated αs1-Casein by Modification with Papain

もっと見る 閉じる

スポンサーリンク