Construction of Mutants of Sphingomonas paucimobilis Defective in Terminal Mannose in the Glycosphingolipid
スポンサーリンク
概要
- 論文の詳細を見る
Mutants of Sphingomonas paucimobilis defective in a part of the carbohydrate moiety of the glycosphingolipid (GSL) were constructed by transposon (Tn5)-insertional mutagenesis. Defective mutants were selected by ELISA using the antibody recognizing the tetrasaccharide-type GSL (GSL-4A) of S.paucimobilis. Eight defective mutants were selected from about 8,000 kanamycin-resistant strains, and seven of them were found to lack the terminal mannose of GSL-4A. The chemical structure of the mutant GSL was investigated, and proved that the rest of the structure was not changed by the mutation.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
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DANBARA Hirofumi
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University
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Kita Katsunori
Pharmaceutical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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KANEKO Aki
Department of Infectious Diseases and Tropical Medicine, Research Institute, International Medical C
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MIYADAI Hidenori
Department of Bacteriology, The Kitasato Institute
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KAWAHARA Kazuyoshi
Department of Bacteriology, The Kitasato Institute
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Matsui Hirotoshi
Department Of Geriatric Medicine University Of Tokyo Hospital
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Kaneko Aki
Department Of Microbiology School Of Pharmaceutical Science Kitasato University
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Kaneko Aki
Department Of Bacteriology The Kitasato Institute
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Kawahara K
Department Of Bacteriology The Kitasato Institute
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Kawahara Kazuyoshi
Department Of Applied Material And Life Science College Of Engineering Kanto Gakuin University
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Miyadai Hidenori
Department Of Bacteriology The Kitasato Institute
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Matsui Hidenori
Laboratory Of Infectious Diseases And Immunology
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Danbara Hirofumi
Department Of Microbiology School Of Pharmaceutical Science Kitasato University
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