One Step Synthesis of Inverted Aspartame Type Sweetener, Ac-Phe-Lys, Using Chemically Modified Chymotrypsin
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概要
- 論文の詳細を見る
To search for techniques of simplified peptide synthesis, benzyloxycarbonyl chymotrpsin was prepared by a water-soluble acylating reagent and used to make Ac-Phe-Lys, an artificial peptide sweetener, which was selected as a target compound. As a result of using chemically modified chymotrypsin, Lys can be coupled directly with Ac-Phe and Ac-Phe-Lys made virtually in one step. Moreover, the total yield from preparation and purification steps for Ac-Phe-Lys was 13%. The value corresponds to that of the chemical synthesis method. On the contrary, enzymatic synthesis using native chymotrypsin cannot reach the level of the new method. It is expected that the method is more effective for simplified peptide synthesis as compared with other methods, especially on a large scale.
- 社団法人日本農芸化学会の論文
- 1999-07-23
著者
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NAKAHARA Kayoko
Department of Food Science and Nutrition, Nara Women's University
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Okai Jiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Okai Hideo
Tokyo Research Laboratories Kowa Company Ltd.
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Oaki Jiro
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University:(present Address)k
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TAMURA Masahiro
Tokyo Research Laboratories, Kowa Company, Ltd.
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Tamura Masahiro
Tokyo Research Laboratories Kowa Company Ltd.
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Nakahara K
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Nakahara Kayoko
Department Of Food Science And Nutrition Nara Women's University
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Nakahara Kayoko
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
関連論文
- Peptide Synthesis in Aqueous Solution. V.^ Properties and Reactivities of (p-Hydroxyphenyl)benzylmethylsulfonium Salts for Direct Benzyl Esterification of N-Acylpeptides^
- Increase of Ceramide in the Liver and Plasma after Carbon Tetrachloride Intoxication in the Rat
- Perfect Enantioselective Hydrolysis of Amino Acid Esters by Modified α-Chymotrypsin
- One Step Synthesis of Inverted Aspartame Type Sweetener, Ac-Phe-Lys, Using Chemically Modified Chymotrypsin