Cloning of Bacillus stearothermophilus ctaA and Heme A Synthesis with the CtaA Protein Produced in Escherichia coli
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概要
- 論文の詳細を見る
The Bacillus stearothermophilus ctaA gene, which is required for heme A synthesis, was found upstream of the ctaBCDEF/caaEABCD gene cluster as in B. subtilis and B. firmus. The deduced protein sequence indicate that CtaA is a 35-kDa intrinsic membrane protein with seven hydrophobic segments. Alignment of CtaA sequences showed conserved residues including histidines that may be involved in heme B binding and substrate binding. Expression of ctaA in E. coli resulted in increased formation of a membrane-bound b-type cytochrome, heme A production, and severe growth inhibition. Furthermore, B. stearothermophilus CtaA produced in E. coli was found to catalyze the conversion of heme O to heme A in vitro.
- 社団法人日本農芸化学会の論文
- 1999-01-23
著者
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Sakamoto Junshi
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology
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Sone Nobuhito
Department of Bioscience, and Bioinformatics, Kyushu Institute of Technology
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Noguchi Satoru
Department Of Neuromuscular Research National Institute Of Neuroscience National Center Of Neurology
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NOGUCHI Shunsuke
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Ky
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Sakamoto J
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Sakamoto Junshi
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Sone Nobuhito
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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HAYAKAWA Akiko
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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UEHARA Tomoko
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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Uehara Tomoko
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Noguchi S
Kyusyu Inst. Technol. Fukuoka Jpn
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Noguchi Shunsuke
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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