Transfer of Pro-R Hydrogen from NADH to Dihydroxyacetonephosphate by sn-Glycerol-1-phosphate Dehydrogenase from the Archaeon Methanothermobacter thermautotrophicus(Biochemistry & Molecular Biology)
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概要
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sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of the sn-glycerol-1-phosphate backbone of archaeal lipids. [4-^3H] NADH that had ^3H at the R side was produced from [4-^3H] NAD and glucose with glucose dehydrogenase (a pro-S type enzyme). The ^3H of this [4-^3H] NADH was transferred to dihydroxyacetonephosphate during the sn-glycerol-1-phosphate dehydrogenase reaction. On the contrary, in a similar reaction using alcohol dehydrogenase (a pro-R type enzyme), ^3H was not incorporated into glycerophosphate. These results confirmed a prediction of the tertiary structure of sn-glycerol-1-phosphate dehydrogenase by homology modeling.
- 2003-07-23
著者
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Sone Nobuhito
Department of Bioscience, and Bioinformatics, Kyushu Institute of Technology
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KOGA Yosuke
Department of Chemistry, University of Occupational and Environmental Health
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Koga Yosuke
Department Of Chemistry School Of Medicine University Of Occupational And Environmental Health
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MORII HIROYUKI
Department of Chemistry, University of Occupational and Environmental Health, Japan
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Koga Y
Department Of Chemistry School Of Medicine University Of Occupational And Environmental Health
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NOGUCHI Shunsuke
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Ky
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Sone Nobuhito
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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Morii Hiroyuki
Department Of Chemistry School Of Medicine University Of Occupational And Environmental Health
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Morii Hiroyuki
Department Of Chemistry University Of Occupational And Environmental Health
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Noguchi Shunsuke
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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