Direct Correlationship between Proton Translocation and Growth Yield : An Analysis of the Respiratory Chain of Bacillus stearothermophilus
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概要
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Thermophilic bacilli contain cytochrome caa_3-type cytochrome caa_3-type cytochrome c oxidase as the main terminal oxidase in the respiratory chain. A mutant strain, named K-17, lacking cytochrome caa_3 and exhibiting very low N, N, N', N'-tetramethyl-p-phenylene diamine oxidase activity, was isolated by random mutation from Bacillus stearothermophilus K1041(Sakamoto, J.et al., FEMS Microbiol.Lett., 143, 151-158, 1996). Comparing this mutant with the parent strain K1041, we obserbed the following differences in energy-yielding properties. (i) K-17 gave an cell yield less than one half of that of the wild type, although the doubling time of K-17 was only a little slower than that of the parent strain. (ii) In cellular respiration, the H^+/O ratio of K-17 was 2.9-3.1, while that of the wild type was 6.1-6.5. (iii) A low concentration of cyanide inhibited endogenous respiration of the wild-type cells partly with a concomitant reduction of the H^+/O ratio to around 3, while it did not significantly affect the respiration rate and the H^+/O ratio of the K-17 cells. (iv) Cytochrome bd-type quinol oxidase seemed to operate in the wild-type cells when a low concentration(below 0.5mM) of cyanide was added, while this enzyme is the main terminal oxidase in K-17. The K-17 cells also contained cytochrome b(o/a)_3-type cytochrome c-551 oxidase. These results demonstrated that the combination of the enzymes involved in the respiratory chain determines the H^+/O ratio of the cell and consequently the growth yield of the bacteria.
- 社団法人日本生物工学会の論文
- 1999-04-25
著者
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Sone Nobuhito
Department of Bioscience, and Bioinformatics, Kyushu Institute of Technology
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Sone Nobuhito
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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TSUKITA Sakura
Department of Biochemical System Engineering, Kyushu Institute of Technology
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SAKAMOTO JUNSI
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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Sakamoto Junsi
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Tsukita S
Department Of Biochemical System Engineering Kyushu Institute Of Technology
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Tsukita Sakura
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
関連論文
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