Over-Expression and Post-Translational Modification of Thermophilic Bacillus Cytochrome c-551 in Bacillus subtilis
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概要
- 論文の詳細を見る
A plasmid for expression of the thermophillic bacterial cytochrome c-551 in Bacillus subtilis was constructed. The transformed cells of B. subtilis 1012 looked slightly pink, and cytochrome c-551 was found in the membrane fraction in amounts as large as 2.5 nmol per mg membrane protein. The cytochrome was extracted with cholate, and then purified by precipitation with polyethylene glycol, ion-exchange chromatography and gel filtration. Analysis of the post-transilation modification of the cytochrome showed that similar events, such as signal peptide cleavage, and modification of the new N-terminal cystein residue with diacrylated glycerol, took place as in the case of thermophilic bacilli, but the blocking of the N-terminus was incomplete in B. subtillis.
- 公益社団法人日本生物工学会の論文
- 1997-09-25
著者
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NOGUCHI Shunsuke
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Ky
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Sone Nobuhito
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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KAI KUMIKO
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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Noguchi Shunsuke
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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Noguchi Shunsuke
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Kai Kumiko
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
関連論文
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