Improved H^+/O Ratio and Cell Yield of Escherichia coli with Genetically Altered Terminal Quinol Oxidases
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概要
- 論文の詳細を見る
Escherichia coli wild-type cells, containing both cytochrome bo-and bd-type terminal oxidases, pumped protons with a H^+/O ratio of 4.5-4.9 upon an oxygen pulse, while mutant cells, deprived of either cytochrome bo (Δcyo) or bd (Δcyd), showed values of 3.5-4.1 or 4.8-5.6, respectively. The cell yield of the cyo-less mutant was about 15% lower than that of the wild-type strain, while that of the cyd-less strain which over-produced cytochrome bo was about 10% higher than that of the wild-type. The simple cyd-less strain without over-production of cytochrome bo showed a high H^+/O ratio, but its cell yield was low and variable from culture to culture. The growth inhibition and accelerated H^+ permeability of the cell membrane of the latter strain seems due to the deletion of cytochrome bd (CydAB), the terminal oxidase having a very low K_m for O_2, which may result in severe stress on the cell. Over-production of cytochrome bo by as much as 0.4 nmol/mg membrane protein could compensate for this defect.
- 社団法人日本生物工学会の論文
- 2002-05-25
著者
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Sakamoto Junshi
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology
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Sone Nobuhito
Department of Bioscience, and Bioinformatics, Kyushu Institute of Technology
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Sakamoto Junshi
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Sone Nobuhito
Department Of Biochemical Engineering And Science Faculty Of Computer Science And System Engineering
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MINOHARA SHINJI
Department of Biochemical Engineering and Science, Kyushu institute of Technology
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Minohara Shinji
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Sakamoto Junshi
Department of Biochemical Engineering and Science, kyushu Institute of Technology
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