Identification of the Replication Region of Streptococcus thermophilus No. 29 Plasmid pST1

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概要

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• The replication region of the 2.77-kilobases (kb) plasmid pST1 from Streptococcus thermophilus No.29 was identified. Deletion derivatives of pST1 were introduced into plasmid-free S. thermophilus No.29 and examined for their ability to replicate autonomously. The nucleotide sequence had one open reading frame encoded for a 315 amino acid protein (Rep). Comparisons with proteins encoded by other Gram-positive bacterial plasmids strongly suggest that the deduced protein (RepS) encoded by pST1 has a replicative role. pST1 also contains a DNA sequence similar to the origin nick sequences of pLP1 or pLAB1000, which initiate plasmid replication at the plus origin. In a maxicell system, E. coli CSR603 carrying pSTUC4 produced a protein that was considered to correspond to the product of the RepS gene. These results strongly suggest that pST1 is replicated following a rolling-circle mechanism via single-stranded DNA intermediates.

• 社団法人日本農芸化学会の論文
• 1993-10-23

著者

• Aoyama K

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Hashiba Honoo

  Technical Research Institute, Snow Brand Milk Products Co., Ltd.,

• Takiguchi Ryuichi

  Technical Research Institute, Snow Brand Milk Products Co., Ltd.,

• Joho Kenichi

  Technical Research Institute, Snow Brand Milk Products Co., Ltd.,

• Aoyama Kenji

  Technical Research Institute, Snow Brand Milk Products Co., Ltd.,

• Hirota Tetsuji

  Technical Research Institute, Snow Brand Milk Products Co., Ltd.,

• Joho Kenichi

  Technical Research Institute Snow Brand Milk Products Co. Ltd.

• Hashiba H

  Snow Brand Milk Products Co. Ltd. Saitama Jpn

• Hirota Tetsuji

  Technical Research Institute Snow Brand Milk Products Co. Ltd.

• Takiguchi R

  Technical Research Institute Snow Brand Milk Products Co. Ltd.

• Takiguchi Ryuichi

  Technical Research Institute Snow Brand Milk Products Co. Ltd.

• Aoyama K

  Technical Research Institute Snow Brand Milk Products Co. Ltd.

• Aoyama Kenji

  Technical Research Institute Show Brand Milk Products Co. Ltd. (present Addresses)technology Develop

関連論文

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• Identification of the Replication Region of Streptococcus thermophilus No. 29 Plasmid pST1
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