Transformation of Lactobacillus helveticus subsp. jugurti with Plasmid pLHR by Electroporation(Microbiology & Fermentation Industry)
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概要
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Lactobacillus helveticus subsp. jugurti was transformed with plasmid, pLHR (8.5 kilobases), by electroporation. The plasmid, pLHR, consists of a cryptic plasmid, pLJ1, from L. helveticus subsp. jugurti, the Escherichia coli vector pBR329, and the erythromycin resistance gene of pAMβ1 from Enterococcus faecalis. Maximum transformation efficiency of 1.3 x 10^4 transformants per μg of DNA was obtained by exposure to a pulse with an exponential decay waveform at 4kV/cm with 25 μF capacitance. The presence of glycine in the growth medium was essential for transformation. Plasmid DNA isolated from transformants had not undergone detectable rearrangements or deletions. In addition, it was found that L. helveticus subsp. jugurti has a restriction and modification system.
- 社団法人日本農芸化学会の論文
- 1990-06-23
著者
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Ishii S
Sapporo Breweries Ltd. Shizuoka Jpn
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Aoyama K
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Hashiba Honoo
Technical Research Institute, Snow Brand Milk Products Co., Ltd.,
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Takiguchi Ryuichi
Technical Research Institute, Snow Brand Milk Products Co., Ltd.,
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Aoyama Kenji
Technical Research Institute, Snow Brand Milk Products Co., Ltd.,
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ISHII Satoshi
Technical Research Institute, Snow Brand Milk Products Co., Ltd.
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Hashiba H
Snow Brand Milk Products Co. Ltd. Saitama Jpn
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Takiguchi R
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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Takiguchi Ryuichi
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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Aoyama K
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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Aoyama Kenji
Technical Research Institute Show Brand Milk Products Co. Ltd. (present Addresses)technology Develop
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