Optimization of Aeration and Agitation Rates to Improve Cellulase-Free Xylanase Production by Thermotolerant Streptomyces sp. Ab106 and Repeated Fed-Batch Cultivation Using Agricultural Waste
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概要
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Thermostable cellulase-free xylanase was produced by Streptomyces sp. Ab106 using agricultural waste, sugar cane bagasse, as the substrate at 50℃ and pH 7.0. The central composite face-centered experimental design was applied to evaluate the optimal agitation and aeration rates in a 5-l fermentor. The highest activity (16.0±0.5 IU/ml) was obtained at an aeration rate of 1 vvm and an agitation rate of 150 rpm (k_L a=351 h^<-1>). Using the repeated fed-batch cultivation technique, the maximum xylanase activity of 32±1 IU/ml was obtained during the second cycle of repeated fed-batch culture.
- 社団法人日本生物工学会の論文
- 2003-03-25
著者
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Poosaran N
Department Of Biotechnology Faculty Of Agro-industry Chiangmai University
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TECHAPUN CHARIN
Department of Biotechnology, Faculty of Agro-Industry, Chiangmai University
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POOSARAN NAIYATAT
Department of Biotechnology, Faculty of Agro-Industry, Chiangmai University
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WATANABE MASANORI
Materials Science and Engineering, Graduate School of Engineering, Hiroshima Kokusai Gakuin Universi
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SASAKI KEN
Materials Science and Engineering, Graduate School of Engineering, Hiroshima Kokusai Gakuin Universi
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Sasaki K
Graduate School Of Engineering Hiroshima Kokusai Gakuin University
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Techapun C
Department Of Biotechnology Faculty Of Agro-industry Chiangmai University
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Techapun Charin
Department Of Biotechnology Faculty Of Agro-industry Chiang Mai University
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Sasaki Ken
Material Science And Engineering Graduate School Of Engineering
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Watanabe Masanori
Materials Science And Engineering Graduate School Of Engineering Hiroshima Kokusai Gakuin University
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