Ca^<2+> Overload Evokes a Transient Outward Current in Guinea-Pig Ventricular Myocytes

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概要

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• There are 2 types of transient outward currents(I_<to>)in the hearts of various mammals : a 4-aminopyridine(4-AP)sensitive K^+ current and a 4-AP resistant Ca^<2+> activated current, carried by Cl-, (referred to as I_<tol> and I_<to2> respectively). However, the I_<to> has been considered to be absent in guinea-pig ventricular myocytes and so this study tested the hypothesis that I_<tol> is generally absent in guinea-pig ventricular myocytes, but I_<to2> appears under the condition of Ca^<2+> overload. Membrane currents were recorded by the whole-cell patch-clamp technique and Ca^<2+> overload was achieved by adding internal, and e1iminating external, Na^+ with subsequent enhancement of Ca^<2+> influx via the Na^+-Ca^<2+> exchange. Under physiological conditions, I_<to> could not be elicited by 300ms-test pulse from-70mV to 0mV(n=32). However, under Ca^<2+> overload, a biphasic current resulting from the overlap of the L-type Ca^<2+> channel current and I_<to> was elicited(n=38). This I_<to> was resistant to 4-AP(3mmol/L, n=30)but sensitive to both anthrancene-9-carboxylic acid(9-AC, 3mmol/L, n=8)and 4, 4'-diisothiocyanatostilbene-2, 2'-disulfonic acid(100μmol/L, n=3). Replacing K^+ with Cs^+ on both sides of the membrane failed to abolish I^<to>(n=38). I_<to> disappeared by lowering the external Cl-(n=3). The amplitude of I_<to> was dependent on that of the L-type Ca^<2+> channel current(n=4). Because Ca^<2+> release from the sarcoplasmic reticulum was prevented by caffeine(5 mmol/L), I_<to> was negligible(n=6). These results suggest that I^<tol> is absent, but Ca^<2+> overload evokes I_<to2> in guinea-pig ventricular myocytes.

• 社団法人日本循環器学会の論文
• 2001-12-20

著者

• Iino Kenji

  Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine

• Watanabe Hiroyuki

  榊原記念病院 心臓血管外科

• Watanabe Hiroyuki

  Second Department of Internal Medicine, Akita University School of Medicine

• Iino Kenji

  Second Department of Internal Medicine, Akita University School of Medicine

• Tosa Shinya

  秋田大学 医学部内科循環器内科学

• SAITO Takashi

  Second Department of Internal Medicine Okayama University Medical School

• Iino Kenji

  Department Of Cardiovascular And Respiratory Medicine Akita University Graduate School Of Medicine

• Watanabe Hiroyuki

  Department Of Cardiovascular And Respiratory Medicine Akita University Graduate School Of Medicine

• Nakajima Ichirota

  Second Department of Internal Medicine, Akita University School of Medicine

• Miura Mamoru

  Second Department of Internal Medicine, Akita University School of Medicine

• Miura Mamoru

  The Second Department of Internal Medicine Akita University School of Medicine

• Miura Mamoru

  The Second Department Of Internal Medicine Akita University School Of Medicine Akita.

• Miura Mamoru

  Second Department Of Internal Medicine Akita University School Of Medicine

• Nakajima Ichirota

  The 2nd Department Of Internal Medicine Akita University School Of Medicine

• Miura Mamoru

  The 2nd Depertment Of Internal Medicine Akita University

• Takahashi Satoru

  The Second Department Of Internal Medicine Akita University School Of Medicine Akita.

• Miura Mamoru

  Second Dept. Of Internal Medicine Akita University

• Sato Tadaya

  Division Of Cardiology Akita Medical Center

• Saito Takashi

  Second Dept.of Internal Medicine Akita University

• Watanabe Hiroyuki

  Second Department Of Internal Medicine Akita University School Of Medicine

• Miura Mamoru

  The 2nd Department Of Intemal Medicine Akita University School Of Medicine

• Miura Mamoru

  Second Dept.of Internal Medicine Akita University

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