せん断応力負荷の初期過程における培養内皮細胞の形態およびF-アクチンフィラメントの変化〔流体工学, 流体機械〕

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The time course changes of F-actin distribution and morphology of cultured bovine aortic endothelial cells under shear flow conditions were analyzed. Fully confluent endothelial cells cultured on glass were subjected to fluid shear stress of 2 Pa. Experiments were performed for one-way flow for 20,40 min, 1,3 and 6 hours. After the flow exposure F-actin filaments were stained with rhodamine-phalloidin. The morphological parameters of endothelial cells (shape index and angle of cell orientation), the appearance frequency of the stress fibers and the angle of stress fiber orientation were measured. F-actin filaments were mainly localized at periphery of the cells, although some filaments were seen as star-like configurations in the center portion under no-flow conditions. In some cells (16%) exposed to shear stress for 20 min, stress fibers which were aligned with cell axes were partly observed. In this stage, however, cell morphological changes were not observed. In many cells (64%) exposed to shear stress for 1 hour, the star-like structure of filaments disappeared in the center portion, and stress fibers appeared. After 6 hours, stress fibers were observed in the most cells (91%) and began to align with flow direction. The cells in which stress fibers appeared significantly elongated, but the cells without stress fibers had kept their polygonal shape. From these results, it is expected that F-actin filaments may play a major role for sensing the level and direction of shear stress and for changing morphology of endothelial cells.
一般社団法人日本機械学会の論文
1998-06-25

せん断応力負荷の初期過程における培養内皮細胞の形態およびF-アクチンフィラメントの変化〔流体工学, 流体機械〕

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