438 ECIS 及び AFM を用いた可逆的不死化ヒト血管内皮細胞のマイクロメカニクス評価

概要

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SV40T(Simian Virus 40 largeT) and Cre-10xP-mediated reversible immotalization technique was applied to human aortic endothelial cells (HAECs). Established immortalized-HAECs (imHAECs) endocytosed acetylated LDL(Low Density Lipoprotein), and PDL(Population Doubling Level) of imHAECs reached more than 80 while that of mormal HAECs (noHAECs) was about 20. Micro-mechanics of imHAECs was evaluated using ECIS(Electric Cell-substrate Impedance Sensing) and AFM(Atomic Force Microscope). imHAECs appeared to have tighter monolayer, and had more developed stress fibers than noHAECs, and elastic modulus of imHAECs was slightly higher than that of noHAECs. These results suggest that imHAECs have superior barrier function compared to noHAECs as well as keep their phenotype of endothelial cell, and have wide variety of applications such as cell source for in vitro study of atherosclerosis, future cell-based therapy for vascular disease of humans, etc.
2003-08-05