凍結保護物質を含む赤血球懸濁液の凝固過程における微細構造に対する不凍たんぱく質の影響 (<小特集>バイオエンジニアリング)

概要

論文の詳細を見る
This study aims at clarifying fundamenentally the influence of antifreeze proteins (AFPs) on the cryopreservation of biological cells. AFPs are antifreeze agents present in Antarctic fishes and some north-temperate fishes, which protect them from freezing. The process of directional solidification of suspensions of human red blood cells was investigated microscopically, using physiological saline including 20%v/v glycerol with and without 20mg/ml AFPs. The red blood cells after freezing and thawing were also observed. Due to the addition of AFPs, the morphology of the ice crystal growing in the suspension changed dramatically from dendritic into spicular. The spiculate ice crystal caused mechanically strong interactions between the ice crystals and the red blood cells, and the resultant complete destruction of the red blood cells. This result suggests that the morphology of the ice crystal and its interaction with the cells are among the factors influencing cell survival through freezing.
一般社団法人日本機械学会の論文
1994-11-25