Structural Alteratlons of DNP Fibers by Irradiation

概要

論文の詳細を見る
Calf thymus deoxyribonucleoprotein solution was gamma-irradiated at a dose of 1,000KR, and negatively stained samples were examined by electron microscopy. The irradiated DNP fibers showed a reduction in their diameter as well as a fragmentation of the fibers. There are a considerable number of studies on the function of deoxyribonucleoprotein (DNP) which is altered by irradiation. As far as the authors are aware, however, no report pertaining to the fine structure of the irradiated DNP is accessible. The present study was undertaken with the hope of demonstrating the structure of the irradiated DNP by electron microscopy. DNP was extracted from calf thymus by the method of Zubay and Doty with some modifications. Final dialysis was carried out against 0.7 mM prosphate buffer solution (pH 6.8) for 48 hours at 4℃. After dialysis, the DNP solution in a Pyrex glass tube, 7 mm in inner diameter, was kept at 0℃, and gamma-irradiated at a dose rate of 500 KR/hour from a cobalt-60 source (2, 000 Ci) to a dose of 1, 000 KR.
日本放射線影響学会の論文