位相差顯微鏡によるヒメコガネの第一成熟分裂の観察

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The course of the 1st spermatocyte division of a beetle, Anamala rufocuprea Motsch., was studied in the living condition by phase-microscopy using the hanging-drop method with fresh material mounted with body fluid. At metaphase, the 1st spermatocyte contains a large amount of mitochondria which are fragmental filaments in shape and distributed surrounding the spindle body forming a think layer (Figs. 1, 9). With the separation of the chromosomes at anaphase, the cell body elongates along the spindle axis. The mitochondria also arrange themselves parallel to the cell axis assuming a form of two solid bundles (Figs. 3, 11; Figs. 4, 12). At telophase the two solid mitochondrial bundles come together. The cleavage furrow always appears across the middle part of the mitochondrial bundle stretching between two daughter nuclei (Figs. 4-6; Figs. 12-14). Following the completion of cell division, the mitochondrial bundle appears as a bridge connecting the daughter cells outside the cytoplasm for a considerable length of time (Figs. 7, 15). The time required for cell division was observed as follows: Metaphase…29+〜49+(min.) Anaphase…17〜26(min.) Telophase…30〜50(min.)
社団法人日本動物学会の論文
1955-02-15