脳室脈絡叢における酵素組織化学的研究 : Na^+, K^+-ATPaseの電顕的免疫組織化学的局在

概要

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The peroxidase-antiperoxidase (PAP) method was employed to determine the ultrastructural localization of Na^+, K^+-adenosinetriphosphatase (ATPase) in normal adult canine choroidal epithelium. The α- and β-subunits of Na^+, K^+-ATPase were prepared from Na^+, K^+-ATPase holoenzyme, which was purified from canine renal outer medulla. Rabbit antisera to both subunits were then produced. The intense immunoreactive electron-dense products were confined along the apical plasmalemma, including that of microvilli of choroidal epithelial cells. This finding is compatible with that of the authors' previous report by light microscopic immunohistochemistry. It can be inferred that the apical border of the epithelium is a very important site for cerebrospinal fluid secretion.
日本脳神経外科学会の論文
1985-11-15